Journal: bioRxiv

Article Title: A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction

doi: 10.1101/2025.08.28.672891

Figure Lengend Snippet: A Representative micrographs of data quantified in B from WT, GBA1 -p.E326K KI, GBA1 -p.L444P KI, and GBA1 KO cells depicting LysoFQ-GBA probe fluorescence (green). Nuclei stained with DAPI (blue); scale bar is 10 µm. B WT, GBA1 -p.E326K KI, GBA1 -p.L444P KI, and GBA1 KO HEK293T cells were stained with LysoFQ-GBA probe to measure lysosomal GCase activity, and the mean GCase activity for each clone is quantified as the sum LysoFQ-GBA spot intensity per nuclei, normalized to the mean of all WT cells. n = 3 independent experiments. C Representative immunoblot of whole cell lysates from HEK293T cells of indicated genotype assessed with antibodies against GCase and actin as a loading control. D Quantification of total cellular GCase enzyme levels, measured via immunoblot & normalized to the levels of actin and the mean signal from WT cells. n = 3 independent experiments. E Representative immunoblot of Lyso-IP samples from HEK293T cells (of indicated genotype) expressing TMEM192-HA x3 tag. Immunoblots were probed using antibodies against GCase, HA, NPC2, and LIMP2. F Quantification of lysosomal GCase levels (normalized per sample to HA band intensity), measured via immunoblotting of Lyso-IP fraction and normalized to the mean of all WT samples. n = 4 independent experiments. G Representative immunoblot of total cell lysates from HEK293T cells of indicated genotype, treated ± Endoglycosidase H (EndoH), and probed using antibodies against GCase and actin as a loading control. H Quantification of the EndoH-resistant GCase band intensity normalized to the total GCase band intensity, from EndoH-treated cell lysates from HEK293T cells of indicated genotype. n = 4 independent experiments. I Representative immuoblots from Strep-Tactin pulldowns performed on lysates from GBA1 KO HEK293T cells transfected with StrepII-tagged GBA1 cDNA for WT GBA1 and GBA1 -p.E326K. Immunoblots were probed using antibodies against LIMP2, GCase and Strep. J Quantification of LIMP2:GCase band intensity ratios, normalized to the WT GBA1/pH 7.3 lysis condition within each replicate, from pulldown fraction of experiment shown in I. n = 4 independent experiments. All bar graphs depict mean ± SEM with individual points representative of data from a single experimental replicate. Unless otherwise noted, all statistics were performed on genotype-level comparisons using one-way ANOVA with Dunnett’s multiple comparison test, where ** = p < 0.0021, *** = p < 0.0002, **** = p < 0.0001.

Article Snippet: Gba E326K mice (and age-matched WT control mice) were obtained from the Jackson Laboratory (strain #036159).

Techniques: Fluorescence, Staining, Activity Assay, Western Blot, Control, Expressing, Transfection, Lysis, Comparison

Journal: bioRxiv

Article Title: A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction

doi: 10.1101/2025.08.28.672891

Figure Lengend Snippet: A Enzymatic activity of purified recombinant GCase (WT or E326K) against the substrate 4-methylumbelliferyl b-glucopyranoside (4-MUG), at an enzyme concentration of 2 nM. Curves depict data fits to Michaelis-Menten kinetics model (top). B Selected enzymatic parameters, derived from Michaelis-Menten kinetics shown in B . C Enzymatic activity of purified recombinant GCase (WT or E326K) against GlcCer in liposomes prepared with either neutral lipids (gray bars) or with 30% BMP (orange bars). Imiglucerase is colored in grey, recombinant WT GCase in blue and E326K in orange. n = 3 independent experiments. All bar graphs depict mean ± SEM with individual points representative of data from a single experimental replicate. D Overlay of size exclusion chromatography profiles of purified WT (blue) and E326K (orange) GCase. Samples were run over a Superdex S200 3.2/100 pre-equilibrated in 20mM sodium acetate pH 5.5 and 150mM NaCl and absorbance at 280nm was recorded. Early elution profile for E326K suggests that protein behaves as a multimer compared to WT GCase. Void volume is expected around 0.9mL confirming E326K is not a soluble aggregate. E Binding affinities measured by SPR for the LIMP2-GCase interaction at both neutral and acidic pHs, with LIMP2 lumenal domain immobilized on the chip. Values correspond to the average of 2 independent replicates, using either a 1:1 (top table) or biphasic (bottom table) binding model. F SEC-MALS analysis of the GCase E326K / LIMP2 complex is consistent with a 2:2 dimeric organization (observed molecular weight ∼ 200 kDa, expected MW for GCase ∼50kDa and LIMP2 extracellular domain ∼45kDa, not accounting for glycosylation). Absorbance trace is shown in blue, and apparent molecular weight is shown in purple. G CryoEM reconstruction of the E326K variant & LIMP2 lumenal domain complex at a resolution of 3.3 Å. Two orthogonal side views of an isosurface rendering of the complex are shown. LIMP2 N- and C-termini are indicated relative to the lysosomal membrane. H Cartoon representation of the E326K variant & LIMP2 complex structure (center). K326 in both E326K variant protomers is shown in red spheres. Overlay of Loop 1 in E326K variant & LIMP2 complex structure with helical (blue) and extended (green) conformations of Loop 1 observed in WT GCase at neutral pH (PDB: 2NT1, chains A and B) and (top left). Close-up view of E326K dimer interface showing that the interface consists primarily of hydrophobic interactions (upper right). Close-up view of interactions forming the E326K variant interface with LIMP2 (bottom left). Close-up view of the electrostatic interactions at the LIMP2 dimer interface (bottom right).

Article Snippet: Gba E326K mice (and age-matched WT control mice) were obtained from the Jackson Laboratory (strain #036159).

Techniques: Activity Assay, Purification, Recombinant, Concentration Assay, Derivative Assay, Liposomes, Size-exclusion Chromatography, Binding Assay, Molecular Weight, Glycoproteomics, Variant Assay, Membrane

Journal: bioRxiv

Article Title: A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction

doi: 10.1101/2025.08.28.672891

Figure Lengend Snippet: A Crystal structure of the GCase E326K variant dimer at 3.1 Å. Overlay of the E326K variant dimer observed in the crystal structure of E326K alone (gold) and the cryoEM structure of the E326K variant & LIMP2 complex (green). B Close-up view of the interactions of residue 326 in GCase structures. In WT GCase (PDB:2NT1, blue) the carboxylic side chain of Glu326 engages in a salt bridge interaction with guanidium group of R329. This electrostatic interaction is lost in both the E326K variant crystal structure (gold) and the cryoEM structure of the E326K variant & LIMP2 complex (green). C Corresponding electron density (mesh representation) for panel B (contoured at 1σ for the WT and E326K crystal structures, and 8σ for the E326K/LIMP2 cryoEM structure), showing that the E326 and R329 sidechains are both well-ordered and well-resolved in WT GCase, while the K326 and R329 sidechains are more disordered in the E326K variant. D Size exclusion chromatography profiles of purified E326K+R329A (purple), E326K+R329E (light red), and E326K+R329A (violet) recombinant enzyme. Elution profiles for WT GCase and E326K variant are shown in blue and orange, respectively. Elution profiles of monomeric and dimeric proteins are indicated with text. E Representative immunoblot from Strep-Tactin pulldowns performed on lysates from GBA1 KO HEK293T cells transfected with StrepII-tagged GBA1 cDNA for WT GBA1 , GBA1 -p.E326K, and E326K+R329D/E/A variants. Immunoblots were probed using antibodies against LIMP2, GCase and Strep. F Quantification of LIMP2:GCase band intensity ratios, normalized to the WT GBA1 transfected condition within each replicate, from pulldown fraction of experiment shown in E . n = 3 independent experiments, statistics performed using one-way ANOVA with Dunnett’s multiple comparison test to WT GCase, where *** = p < 0.0002, **** = p < 0.0001. All bar graphs depict mean ± SEM with individual points representative of data from a single experimental replicate.

Article Snippet: Gba E326K mice (and age-matched WT control mice) were obtained from the Jackson Laboratory (strain #036159).

Techniques: Variant Assay, Residue, Size-exclusion Chromatography, Purification, Recombinant, Western Blot, Transfection, Comparison

Journal: bioRxiv

Article Title: A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction

doi: 10.1101/2025.08.28.672891

Figure Lengend Snippet: A Heatmaps depicting log 2 fold changes (from WT mean) in the abundance of all detected GCase substrates from whole-cell and Lyso-IP fractions taken from WT, GBA1 -p.E326K KI, GBA1 -p.L444P KI, and GBA1 KO HEK293T cells. B The fold change (over WT mean) of glucosylsphingosine (GlcSph) levels in Lyso-IP samples from HEK293T cells of the indicated genotype. C Schematic depicting the major lipids and key enzymes/protein co-factors in the lysosomal glycosphingolipid degradation pathway proximal to GCase. D Heatmaps showing the log 2 fold change (from WT mean) of glycosphingolipids (from pathway shown in c) in both whole-cell and Lyso-IP fractions taken from HEK293T cells of indicated genotype. Only lipids with a fold change of E326K vs WT that reached nominal significance (p < 0.1) in either the whole-cell or Lyso-IP fractions are included. E Fold change (over WT mean) of BMP(22:6/22:6) levels in Lyso-IP samples from HEK293T cells of the indicated genotype. F Fold change (over WT mean) of glucosylsphingosine levels in whole cell samples from HEK293T cells of the indicated genotype, treated with imiglucerase as indicated. G & H Fold change (over WT mean) of BMP(22:6/22:6) (G) or GB3(d18:1/18:0) (H) levels in whole cell samples from HEK293T cells of the indicated genotype, treated with imiglucerase as indicated. All bar graphs show mean ± SEM with individual points representative of data from a single experimental replicate. Data in A-B, and D-E are from n = 4 independent replicates. Data in F-H are from n = 5 independent replicates. For A and D, statistical tests were performed on genotype-level comparisons using robust linear model as described in methods and significance threshold was set at FDR adjusted P value < 0.05. All other statistical tests were performed on genotype-level comparisons using one-way ANOVA with Dunnett’s multiple comparison test (B and E), or Tukey’s multiple comparisons test (F-H), where * = p < 0.0332, *** = p < 0.0002, **** = p < 0.0001.

Article Snippet: Gba E326K mice (and age-matched WT control mice) were obtained from the Jackson Laboratory (strain #036159).

Techniques: Comparison

Journal: bioRxiv

Article Title: A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction

doi: 10.1101/2025.08.28.672891

Figure Lengend Snippet: A Venn diagram showing overlap in the identity of proteins with significantly altered abundance in lysosomes isolated from GBA1 -p.E326K KI, GBA1 -p.L444P KI HEK293T, and GBA1 KO cells, relative to lysosomes isolated from WT cells. B Volcano plots showing differential abundance of proteins identified in Lyso-IP samples from indicated GBA1 variant or KO cells compared to WT cells. Proteins with significantly changed abundance (p < 0.05; absolute log 2 FC ≥ 0.5) are highlighted in red or blue for increased or decreased abundance, respectively. Proteins identified as mitochondrial in origin in the MitoCarta 3.0 database are highlighted in purple. C The number of proteins identified in each GBA1 variant or KO genotype belonging to the top ten most significant GO: Cell Component terms associated with the subset of significantly depleted proteins in E326K (vs WT) lysosomes. D Oxygen consumption rate (OCR) of HEK293T cells of indicated genotype, measured using Seahorse XF Cell Mito Stress Test assay. n = 3 independent experiments. E HEK293T cells of indicated genotype were stained with TMRM and MitoTracker Green, and the ratio of the sum (per nuclei) TMRM fluorescence to MitoTracker Green fluorescence, normalized to the mean of all WT cells, is plotted. n = 3 independent experiments. F Heatmap of the relative abundance of all identified glycosphingolipid hydrolases (related to the pathway shown in 2c) is shown as log 2 fold change (from WT mean) for both whole-cell and Lyso-IP samples from HEK293T cells of indicated genotype. Analytes that were not detected in a sample are shaded gray. G Representative immunoblot of Lyso-IP samples from HEK293T cells (of indicated genotype) expressing TMEM192-HA x3 tag. Immunoblots were probed with antibodies against GLA, GM2A, SGSH, and HA as a loading and lysosomal isolation control. H Quantification of lysosomal GLA levels (normalized per sample to HA band intensity), measured via immunoblotting of Lyso-IP fraction & normalized to mean of all WT samples. n = 3 independent experiments. Bar graphs in E and H show mean ± SEM with individual points representative of data from separate experimental replicates. All proteomics data (A-C, F) is from n = 3 independent experiments. Unless otherwise noted, all statistical tests were performed on genotype-level comparisons using robust linear model as described in methods and significance threshold was set at FDR adjusted P value < 0.05. Statistics in E and H were performed on genotype-level comparisons using one-way ANOVA with Dunnett’s multiple comparison test, where * = p < 0.0332, ** = p < 0.0021, **** = p < 0.0001.

Article Snippet: Gba E326K mice (and age-matched WT control mice) were obtained from the Jackson Laboratory (strain #036159).

Techniques: Isolation, Variant Assay, Staining, Fluorescence, Western Blot, Expressing, Control, Comparison

Journal: bioRxiv

Article Title: A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction

doi: 10.1101/2025.08.28.672891

Figure Lengend Snippet: A Lysosomal GCase activity, measured in WT, GBA1 -p.E326K, and GBA1 KO human iMicroglia stained with LysoFQ-GBA, is quantified as sum LysoFQ-GBA spot intensity per nuclei, normalized to the mean of all WT cells. n = 3 independent experiments. B Representative immunoblot showing GCase levels in human iMicroglia of indicated genotype. C Quantification of total cellular GCase enzyme levels (normalized to actin) in human iMicroglia of indicated genotype, measured via immunoblot & normalized to mean of all WT cells. n = 3 independent experiments. D Heatmap of whole-cell log 2 fold change (from WT mean) abundance of all detected GCase substrates from human iMicroglia of indicated genotype. Shaded cells indicate values capped at log 2 fold change = 5. E Fold change (over WT mean) of glucosylsphingosine levels in whole-cell samples from human iMicroglia of the indicated genotype. F Heatmap of log 2 fold change (from WT mean) of BMPs & related species from human iMicroglia of indicated genotype. G Heatmap of log 2 fold change (from WT mean) of glycosphingolipids from human iMicroglia of indicated genotype. For F and G, only species with a fold change of E326K vs WT that reached nominal significance (p < 0.1) are included. Lipidomics analysis (D-G) was done using a robust linear model as described in methods and significance threshold was set at FDR adjusted p value < 0.05, from n = 3 independent experiments. Statistics in A, C, and E, were performed on genotype-level comparisons using one-way ANOVA with Dunnett’s multiple comparison test, where * = p < 0.0332, **** = p < 0.0001.

Article Snippet: Gba E326K mice (and age-matched WT control mice) were obtained from the Jackson Laboratory (strain #036159).

Techniques: Activity Assay, Staining, Western Blot, Comparison

Journal: bioRxiv

Article Title: A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction

doi: 10.1101/2025.08.28.672891

Figure Lengend Snippet: A Whole blood-derived GCase activity (µmol/L/hr) is plotted in GBA1 -p.E326K carriers (n = 19), GBA1 pathogenic variant carriers (n = 14), and subjects who do not carry either (n = 346). B and C The levels of selected plasma metabolites (adjusted area ratio), glucosylsphingosine ( B ) and GB3 (d18:1/24:0) ( C ), plotted from GBA1 -p.E326K carriers (n = 14), GBA1 pathogenic variant carriers (n = 159), and subjects who do not carry either (n = 410). P-values are derived from linear models comparing GBA1 -p.E326K carriers or GBA1 pathogenic variant carriers to non-carriers, adjusted for age, sex, PD case status, and 3 principal components derived from genome-wide WGS data (see Methods). Gray circles denote controls and colored circles (blue, orange, or red, for non-carriers, p.E326K carriers, and pathogenic variant carriers, respectively) denote PD cases.

Article Snippet: Gba E326K mice (and age-matched WT control mice) were obtained from the Jackson Laboratory (strain #036159).

Techniques: Derivative Assay, Activity Assay, Variant Assay, Clinical Proteomics, Genome Wide